Rapid screening of Salmonella entericaserovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles

Hong, Yang; Liu, Tongrui; Lee, Margie D; Hofacre, Charles L; Maier, Marie; White, David G; Ayers, Sherry; Wang, Lihua; Berghaus, Roy; Maurer, John J

doi:10.1186/1471-2180-8-178

Table 2 Comparison of multiplex PCR to serotyping for identifying Salmonella O alleles B; C1; C2; D1 or E1; H1 alleles i; g,m; r or z₁₀;and H2 alleles 1,2 or e,n,x

From: Rapid screening of Salmonella entericaserovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles

										Phase 1 PCRs				Phase 2 multiplex PCR
Antigenic Formula					O muliplex PCR					i/g,m multiplex PCR		r/z₁₀multiplex PCR
O	H1	H2	S. enterica Serovars	Animal Origin ( n )¹	B	C1	C2	D1	E1	i	g,m	r	z₁₀	1,2	e,n,x
A	a	1,5	Paratyphi A	1 (1)	0	0	0	1	0	0	0	0	0	1	0
B	b	1,2	Paratyphi B	1 (1)	1	0	0	0	0	0	0	0	0	1	0
B	e,h	1,2	Saintpaul	1(1)	1	0	0	0	0	0	0	0	0	1	0
B	e,h	1,5	Reading	1(2)	2	0	0	0	0	0	0	0	0	1	0
B	f,g	-	Derby	1(1)	1	0	0	0	0	0	1	0	0	0	0
B	i	1,2	Typhimurium	1, 4–6 (74)	74	0	0	0	0	74	0	0	0	74	0
B	l,v	1,7	Bredeney	1(1)	1	0	0	0	0	0	0	0	0	1	0
B	1,v	e,n,z₁₅	Brandenburg	1(2)	2	0	0	0	0	0	0	0	0	0	2
B	b	-	Java	6(1)	1	0	0	0	0	0	0	0	0	0	0
B	e,h	e,n,x	Chester	1(2)	2	0	0	0	0	0	0	0	0	0	2
B	f,g,s	-	Agona	1 (1)	1	0	0	0	0	0	1	0	0	0	0
B	r	1,2	Heidelberg	1, 3–6(24)	24	0	0	0	0	0	0	24	0	24	0
B	z	1,5	Kiambu	1(1)	1	0	0	0	0	0	0	0	0	1	0
B	z	1,7	Indiana	1(2)	2	0	0	0	0	0	0	0	0	2	0
B	z₁₀	1,2	Haifa	6 (1)	1	0	0	0	0	0	0	0	1	1	0
C1	b	l,w	Ohio	1(1)	0	1	0	0	0	0	0	0	0	0	0
C1	c	1,5	Choleraesuis	1, 6(6)	0	6	0	0	0	0	0	0	0	6	0
C1	c	1,5	Paratyphi C	1 (1)	0	1	0	0	0	0	0	0	0	1	0
C1	d	l,w	Livingstone	6(1)	0	1	0	0	0	0	0	0	0	0	0
C1	g,m,s	-	Montevideo	1, 5(12)	0	12	0	0	0	0	12	0	0	0	0
C1	k	1,5	Thompson	1(1)	0	1	0	0	0	0	0	0	0	1	0
C1	m,t	-	Oranienburg	1(1)	0	1	0	0	0	0	1	0	0	0	0
C1	z₂₉	-	Tennessee	1(1)	0	1	0	0	0	0	0	0	0	0	0
C1	e,h	e,n,z₁₅	Braenderup	1(2)	0	2	0	0	0	0	0	0	0	0	2
C1	r	1,5	Infantis	1(2)	0	2	0	0	0	0	0	2	0	2	0
C1	z₁₀	e,n,z₁₅	Mbandaka	1(14)	0	14	0	0	0	0	0	0	14	0	14
C1	z₂₈	-	Lille	1(1)	0	1	0	0	0	0	0	0	0	0	0
C2	d	1,2	Muenchen	5(3)	0	0	3	0	0	0	0	0	0	3	0
C2	e,h	1,2	Newport	4,5(1)	0	0	1	0	0	0	0	0	0	1	0
C2	i	z₆	Kentucky	1(24)	0	0	24	0	0	24	0	0	0	0	0
C2	z₁₀	e,n,x	Hadar	1 (10)	0	0	10	0	0	0	0	0	10	0	10
D1	a	1,5	Miami	5(1)	0	0	0	1	0	0	0	0	0	1	0
D1	a	1,5	Sendai	5(1)	0	0	0	1	0	0	0	0	0	1	0
D1	g,m	-	Enteritidis	1(20)	0	0	0	20	0	0	20	0	0	0	0
D1	g,p	-	Dublin	2, 6(3)	0	0	0	3	0	0	3	0	0	0	0
D1	l,v	1,5	Panama	1 (1)	0	0	0	1	0	0	0	0	0	1	0
D1	-	-	Gallinarum	1(4)	0	0	0	4	0	0	4	0	0	0	0
D1	f,g,t	-	Berta	1 (2)	0	0	0	2	0	0	2	0	0	0	0
D1	l,z₂₈	1,5	Javiana	1 (1)	0	0	0	1	0	0	0	0	0	1	0
E1	e,h	1,5	Muenster	1(2)	0	0	0	0	2	0	0	0	0	2	0
E1	l,v	1,7	Give	1(2)	0	0	0	0	2	0	0	0	0	2	0
E1	e,h	1,6	Anatum	1, 5 (4)	0	0	0	0	4	0	0	0	0	4	0
E1	l,v	1,6	London	1(2)	0	0	0	0	2	0	0	0	0	2	0
		Total		239	114	43	38	34	10	98	44	26	25	135	30
		False Positives			0	0	0	1	0	0	24	0	0	30	18
		False Negatives			0	0	0	0	0	0	0	0	0	0	0
		Kappa²			1.0	1.0	1.0	0.98	1.0	1.0	0.58	1.0	1.0	0.75	0.54

¹1 = poultry; 2 = bovine; 3 = swine, 4 = other (includes dog, heron, horse, opossum, parrot, rabbit, and snake); 5 = human; and 6 = unknown. Numbers in parentheses indicate the numbers of isolates for each serovar.
²Agreement between PCR allelotyping and conventional serotyping results

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