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Figure 2 | BMC Microbiology

Figure 2

From: Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract

Figure 2

(A) Flow diagram for the expression of the bilEAB operon, indicating the listerial bilE operon (B) RT-PCR analysis of the heterologous expression of the bilEAB bile resistance loci in B. breve and L. lactis (a) Control PCRS using cDNA template and the 16S RNA specific primers Bif16SF, Bif16SR, Lac16SF and Lac16SR to confirm the presence of the correct template. (b) bilE specific primers RTPCR F and RTPCR R amplify a 500 bp fragment from both UCC3003-bilE+ and NZ9000-bilE+ cDNA, while as expected no product was obtained when the cDNA of the control strains UCC3003-bilE- and NZ9000-bilE- were used as template.

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BMC Microbiology

ISSN: 1471-2180

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