Table 3 PCR amplification conditions used in the present study.
PCR No | Initial denaturation | Denaturation | Annealing | Extension | No of cycles | Final extension |
|---|---|---|---|---|---|---|
1 | 95°C; 15 min | 95°C; 30 s | 56°C; 30 s | 72°C; 1 min | 30 | 72°C; 10 min |
2 | 95°C; 15 min | 95°C; 30 s | 62°C; 30 s | 72°C; 1 min | 30 | 72°C; 10 min |
3 | 95°C; 15 min | 95°C; 30 s | 55°C; 30 s | 72°C; 1 min | 30 | 72°C; 10 min |
4 | 95°C; 15 min | 95°C; 30 s | 57°C; 30 s | 72°C; 1 min | 37 | 72°C; 10 min |
5 | 95°C; 15 min | 95°C; 30 s | 65°C, 30 s | 72°C; 30 s | 5 | 72°C; 10 min |
95°C; 30 s | 60°C; 30 s | 72°C; 30 s | 25 | |||
95°C; 30 s | 55°C; 30 s | 72°C; 30 s | 5 |