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Figure 9 | BMC Microbiology

Figure 9

From: Sulphur metabolism and cellulase gene expression are connected processes in the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei)

Figure 9

Northern analysis of transcription of lim1, cbh1 and env1 in constant light (LL; 1800 lux, 25 μmol photons m-2s-1) or constant darkness (DD). Strains were cultivated for 72 or 96 hours on conventional Mandels Andreotti minimal medium with 1% (w/v) microcrystalline cellulose as sole carbon source. Met indicates addition of 5 mM methionine to the culture. 20 μg of total RNA were loaded per lane, a hybridization with 18S rRNA is given as a loading control. Transcript abundance was measured by densitometric scanning and normalization to the 18S rRNA loading control.

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