Figure 8From: Sulphur metabolism and cellulase gene expression are connected processes in the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei)Northern analysis of transcription for analysis of light response of lim1. Transcription was analyzed in constant darkness (preculture, DD) and after illumination (1800 lux, 25 μmol photons m-2s-1) for 15, 30 or 45 minutes in the wild-type strain QM9414 and a strain lacking the PAS-domain of the light regulatory protein ENVOY (Schmoll et al., 2005). Strains were cultivated on conventional Mandels Andreotti minimal medium with 1% (w/v) glycerol as carbon source. 20 μg of total RNA were loaded per lane, a hybridization with 18S rRNA is given as a loading control.Back to article page