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Figure 7 | BMC Microbiology

Figure 7

From: Sulphur metabolism and cellulase gene expression are connected processes in the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei)

Figure 7

Northern analysis of transcription of lim1 , cbh1 and env1 in constant light (LL; 1800 lux, 25 μmol photons m-2s-1) or constant darkness (DD). (A) Strains were cultivated for 72 hours on Mandels Andreotti minimal medium lacking sulphate with 1% (w/v) microcrystalline cellulose as sole carbon source and supplemented with 0.05, 0.25 or 5 mM methionine as specified with the samples. (B) Strains were cultivated for the time indicated with the samples on conventional Mandels Andreotti minimal medium with 1% (w/v) microcrystalline cellulose as sole carbon source, which comprises approximately 12 mM sulphate and 0.25 mM methionine due to the addition of peptone. 20 μg of total RNA were loaded per lane, a hybridization with 18S rRNA is given as a loading control.

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