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Table 2 Degradation of S. pneumoniae Biofilms with DNase treatment.

From: Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates

Strain Pulmozyme concentration [% Reduction]
  0 (μg ml-1) 1 (μg ml-1) 100 (μg ml-1) 1000 (μg ml-1)  
Biomass (μm 3 /μm 2 )
Low BFI      
BS68 (9V) 0.20 (0.024) 0.08 (0.005)** 0.04 (0.003)** 0.01 (0.001)** [95.0]
BS71 (3) 0.11(0.013) 0.03 (0.004)** 0.022 (0.005)** 0.02 (0.007)** [81.8]
BS73 (6A) 0.51(0.065) 0.19 (0.019)** 0.08 (0.008)** 0.04 (0.003)** [92.2]
High BFI      
BS69 (14) 0.59 (0.126) 0.26 (0.039)* 0.19 (0.020)** 0.14 (0.021)** [76.3]
BS72 (23F) 2.97 (0.278) 1.50 (0.190)** 0.63 (0.083)** 0.24 (0.027)** [92.0]
BS75 (19F) 0.42 (0.041) 0.38 (0.028) 0.18 (0.020) ** 0.14 (0.017)** [66.7]
Average thickness (μm)
Low BFI      
BS68 (9V) 0.23 (0.032) 0.08 (0.006)** 0.03 (0.003)** 0.01 (0.002)** [95.7]
BS71 (3) 0.19 (0.048) 0.03 (0.017)** 0.003 (0.000)** 0.004 (0.001)** [94.7]
BS73 (6A) 0.46 (0.068) 0.16 (0.020)** 0.04 (0.005)** 0.02 (0.002)** [95.7]
High BFI      
BS 69 (14) 0.46 (0.068) 0.16 (0.020)** 0.04 (0.005)** 0.02 (0.002)** [91.3]
BS72 (23F) 5.23 (0.567) 2.23 (0.404)** 0.43 (0.073)** 0.15 (0.027)** [97.1]
BS75 (19F) 0.39 (0.039) 0.26 (0.024)** 0.12 (0.017)** 0.058 (0.006)** [85.1]
Maximum thickness (μm)
Low BFI      
BS68 (9V) 3.6 (0.147) 3.23 (0.091) 2.44 (0.112)** 2.72 (0.275)** [24.4]
BS71 (3) 5.2 (0.169) 3.72 (0.219)** 2.32 (0.099)** 2.00 (0.148)** [61.5]
BS73 (6A) 5.28 (0.249) 3.2 (0.082)** 2.56 (0.110)** 2.56 (0.073)** [51.5]
High BFI      
BS 69 (14) 7.12 (0.301) 4.72 (0.343)** 4.24 (0.193)** 3.6 (0.159)** [49.4]
BS72 (23F) 10.08 (0.653) 4.96 (0.331)** 3.36 (0.160)** 3.68 (0.309)** [63.5]
BS75 (19F) 4.20 (0.265) 3.84 (0.198) 3.04 (0.110)** 2.24 (0.110)** [44.3]
  1. Value = average (n = 10)
  2. Percent reduction of biofilm with highest dose of Pulmozyme.
  3. (Standard error) * p < 0.05; ** p < 0.01