Figure 5From: Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolatesCapsule expression during biofilm growth conditions in selected strains. Fig. 5A. cpsA, the first gene in the pneumococcal capsule operon is expressed in each isolate over 100-fold relative to R6, an unencapsulated strain (hatched bars), but is downregulated when pneumococcal strains are grown as biofilms (solid bars). Fig. 5B. Immunostaining with anti-capsule specific antibody labeled with a secondary Texas red anti-rabbit antibody, shows that pneumococci express type specific capsule in the biofilm despite cpsA downregulation. Scale bar = 10 μm.Back to article page