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Table 1 Strains, plasmids, and primers used in this study.

From: Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis

Strain, plasmid,
or primer
Description Source or
Reference
   F. tularensis   
LVS F. tularensis subsp. holartica live vaccine strain Karen Elkins
1581d LVS with FTL_1581 disruption, HygR This study
1664d LVS with FTL_1664 (deoB) disruption, HygR This study
   E. coli   
XL10-Gold Δ(mcrA)183 Δ (mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F' proAB lacIqZDM15 Tn10 (TetR) Amy CamR] Stratagene
DH5α F-φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1 Invitrogen
JM109 endA1, recA1, gyrA96, thi, hsdR17 (rk-, mk+), relA1, supE44, Δ(lac-proAB), [F' traD36, proAB, laqIqZΔM15] Promega
Plasmids   
   pFNLTP8 Francisella shuttle plasmid, ApR, KmR [63]
   pRK2013 Helper plasmid for triparental mating, KmR [64]
   pMP615 Francisella shuttle plasmid, HygR [65]
   pMQ131hyg F. tularensis suicide vector, pBBR1 ori, oriT, contains the hyg cassette driven by the groEL promoter from pMP615, oriT, KmR, HygR This study
   pMQ131hyg1581d pMQ131hyg with the central 560 base pair region of FTL_1581 This study
   pMQ131hyg1664d pMQ131hyg with the central 900 base pair region of FTL_1664 (deoB) This study
   pFTL_1581 Broad-host-range vector, pC194 ori, contains cloned FTL_1581 along with 600 base pairs upstream and 100 base pairs downstream of this gene, complementing plasmid, ApR, CamR This study
   pF8AX pFNLTP8 Δnpt, ApR This study
   pF8CAT pF8AX with cat, ApR, CamR This study
   pFTL_1664 pF8CAT with cloned FTL_1664 (deoB) along with 600 base pairs upstream and 100 base pairs downstream of this gene, complementing plasmid This study
Primers   
   1581_560F 5'-ATGGATCCTGAGCTAAATGATGCTTTAGTATCTC-3' Invitrogen
   1581_560R 5'-ATGGTACCAAGACGACATAGCCACG-3' Invitrogen
   1664_900F 5'-ATGGATCCGAACCTGGAGCAGTTGAAT-3' Invitrogen
   1664_900R 5'-ATGGTACCTAAGAAAGTTGCGGAATATAATAGATG-3' Invitrogen
   1581_clone_up 5'-GATCGGATCCAGGTCAATCAGGAGTTGG-3' Invitrogen
   1581_clone_down 5'-GATCGGTACCCACCTATTTGAATTAAAAAGAAGTTTATACAC-3' Invitrogen
   1664_clone_up 5'-GATCGGATCCGATGGCTATGGTATATCTTCGG-3' Invitrogen
   1664_clone_down 5'-GATCGGTACCACCGAGAGAATTTCTCGC-3' Invitrogen
   F8AgeI 5'-CATTAGACCGGTGCGAAACGATCCTCATCCTGTC-3' Invitrogen
   F8XhoI 5'-Phosphorylated – CATTAGCTCGAGGGAAGAGTATGAGTATTCAAC-3' Invitrogen
   XhoICAT 5'-CATGCTCGAGTTATAAAAGCCAGTCATTAGGCC-3' Invitrogen
   AgeICAT 5'-CATGACCGGTATGAACTTTAATAAAATTGATTTAGACAATTGG-3' Invitrogen
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