Global Francisella gene expression changes induced by mammalian body temperature. Genes that were identified as statistically significant by J5 scores across three independent microarray experiments were subjected to hierarchical clustering. Fluorescence intensities were standardized by the minimum mean ratio array normalization followed by log2 transformation. Values were clustered using the Pearson correlation in GenePattern by independently inputting individual data from each experiment. Oligonucleotides testing individual LVS ORFs are in duplicate on each array and the results are displayed as two columns per experiment in GenePattern. The 95 induced genes and 125 repressed genes clustered together in this GenePattern output, validating the J5 statistical analysis from GEDA. (B).