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Figure 2 | BMC Microbiology

Figure 2

From: Determination of the mimic epitope of the M-like protein adhesin in swine Streptococcus equi subsp. zooepidemicus

Figure 2

Characterization of purified rSzP protein binding to HEp-2 cells by ELISA. (A) Binding of biotinylated S. zooepidemicus whole cells, rSzP protein, and a fetuin control to HEp-2 monolayers. (B) Dose-dependent binding of rSzP to HEp-2 cells. (C) Inhibition of S. zooepidemicus binding to HEp-2 monolayers following pretreatment of bacteria with rabbit polyclonal anti-SzP antibodies. (D) No inhibition of HEp-2 binding with control rabbit serum. (E) Inhibition of S. zooepidemicus binding to HEp-2 monolayers following pretreatment of bacteria with monoclonal antibodies against SzP. For all experiments, absorbance values are means from representative experiments performed in triplicate. Error bars show the range of absorbance values.

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BMC Microbiology

ISSN: 1471-2180

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