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Table 3 Bacterial strains and plasmids used in this study

From: Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis

Strain/Plasmid Relevant characteristics Reference
E. colistrains   
S17.1 TpR SmR, recA, thi, pro, hsdR-M+ RP4: 2-Tc:Mu: Km Tn7 λ pir [30]
Brucella strains   
B. abortus RB51 RifR, rough mutant of 2308 [31]
B. melitensis 16M-N Spontaneous NalR mutant of ATCC 23456 This study
B. ovis BOC 22 Rough field strain, CO2 dependent This study
B. pinnipedialis B2/94 Brucella marine strain isolated from common seal, CO2 dependent [32]
Plasmids   
pGBG1 CmR, transposon trap derivative of pBBR-MCS1 [20]
  1. CmR: Chloramphenicol resistant, NalR: Nalidixic acid resistant, RifR: Rifampicin resistant, TpR: Trimethoprim resistant, SmR: Streptomycin resistant,