Table 4 Carotenoid composition of wild-type and transformant X. dendrorhous strains in ppm
Carotenoid | UCD 67–385 wt-strain | T13 (crtRΔBsiWI::hph/crtR+) | CBS-6938 wt-strain | CBSTr (crtRΔNdeI::hph) |
|---|---|---|---|---|
astaxanthin | 102.0 ± (4.8) | 24.0 ± (3.6) | 152.0 ± (8.5) | ND |
phoenicoxanthin | 7.0 ± (0.4) | 8.0 ± (0.4) | 8.0 ± (0.5) | ND |
canthaxanthin | 1.0 ± (0.1) | 3.00 ± (0.06) | 1.0 ± (0.1) | ND |
hydroxy-keto-γ-carotene | 6.0 ± (0.2) | 1.00 ± (0.06) | ND | ND |
HO-keto-torulene | ND | ND | 6.0 ± (1.1) | ND |
keto-γ-carotene | 9.0 ± (0.7) | 11.0 ± (0.7) | 8.0 ± (1.2) | 1.0 ± (0.3) |
HO-echinenone | ND | ND | 2.0 ± (0.4) | 1.0 ± (0.4) |
echinenone | 5.0 ± (0.6) | 16.0 ± (0.5) | 5.0 ± (0.7) | 8.0 ± (1.2) |
lycopene | ND | 1.0 ± (0.01) | ND | ND |
neurosporene | ND | 1.00 ± (0.02) | ND | ND |
γ-carotene | 1.0 ± (0.1) | 2.00 ± (0.05) | 1.0 ± (0.1) | 3.0 ± (0.2) |
β-carotene | 9.0 ± (1.7) | 50.0 ± (3.7) | 9.0 ± (1.5) | 114.5 ± (6.6) |
phytoene | 0.4 ± (0.3) | 4.2 ± (1.9) | 3.3 ± (1.2) | 1.0 ± (0.4) |
NI (25 min)* | ND | 1.0 ± (0.1) | ND | 1.0 ± (0.1) |
NI (34 min)* | ND | 2.00 ± (0.04) | ND | 4.0 ± (0.9) |
Total carotenoids | 139.0 ± (3.2) | 122.8 ± (8.0) | 195.3 ± (8.0) | 131.9 ± (7.5) |