Table 2 Plasmids used in this work
Plasmid | Genotype or relevant features | Source of reference |
|---|---|---|
pBluescript SK- | ColE1 ori; AmpR; cloning vector with blue-white selection | Stratagene |
pBAD33 | p15 ori; CamR; cloning vector | Beckwith Lab. |
YIp5 | S. cerevisiae/E. coli cloning vector. pMB1 ori; AmpR; TETR; URA3. | ATCC 37061 |
pET101/D-TOPO | ColE1 ori; AmpR; expression vector | Invitrogene |
pDS1B | pBAD33 vector carrying the carotenogenesis genes crtE, crtB, crtI and crtY and crtX from Erwinia uredovora [40]. | This work |
pPR52.P.44 | YIp5 bearing an insert of 12.8 kb that contains the crtR gene from base n° 88 of exon 1. | This work |
pCPR1.3 | pBluescript SK- bearing the DNA fragment from site BamHI 4,529 to BamHI 9,895 (Figure 1). pCPR1.3 was the crtR deletion platform. | This work |
pcXDA.44.29 | pBluescript SK- bearing the crtR gene cDNA. The cDNA measures 2,680 bp. | This work |
pPR32.AA.51 | YIp5 bearing an insert of 4.5 kb that contains the crtR gene up to base n° 87 of exon 1. | This work |
pCPRB6 | pBluescript SK- bearing an insert of 5.9 kb that contains the complete genomic version of crtR gene. Isolated from a SalI partial genomic library. | This work |
pBsiWIΔcrtR::hph | pCPR1.3 BsiWI deletion and hph cassette insertion. | This work |
pNdeIΔcrtR::hph | pCPR1.3 NdeI deletion and hph cassette insertion. | This work |