Table 1 E. coli and X. dendrorhous strains used in this work
Strains | Genotype or relevant features | Reference |
|---|---|---|
E. coli | ||
DH-5α | F- φ80d lacZΔM15Δ(lacZY-argF)U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1 | [18] |
DS1B | β-carotene producer strain with a yellow phenotype which corresponds to BL21-Gold strain carrying pDS1B | [6] |
X. dendrorhous | ||
UCD 67–385 | ATCC 24230, wild-type | ATCC |
CBS-6938 | ATCC 96594, wilt-type | ATCC |
T13 | (crtRΔBsiWI::hph/crtR+). Heterozygote transformant from UCD 67–385 parental wild-type strain, containing an allele of the crtR gene with a BsiWI deletion and a hygromycin B resistance cassette. | This work |
CBSTr | (crtRΔNdeI::hph). Transformant from CBS-6938 parental wild-type strain. β-carotene accumulating mutant. | This work |