Figure 6

Bacterial levels in IJ stage nematodes. IJ nematodes were harvested from lipid agar plates inoculated with TT01, BMM800 (ΔflgG) or BMM802 (ΔmotAB). Individual IJs were crushed and the homogenate was plated on LB (Rif) plates to determine the bacterial population per IJ (cfu/IJ). Three independent overnight cultures for each strain were prepared and each overnight was inoculated onto 4 lipid agar plates. After 21 days incubation at 25°C 10 IJs from each plate (n = 120 for each bacterial strain) were homogenised and the number of cfu/IJ was determined by plating the homogenate on LB agar (Rif). Any nematodes that were not colonised were not included in further analyses. Therefore, for IJs grown on WT bacteria n = 100, for IJs grown on the ΔflgG mutant n = 102 and for IJs grown on the ΔmotAB mutant n = 98. The data from these nematodes was analysed and compared to WT using the non-parametric Mann-Whitney U test and the appropriate P values are shown. The data is presented using a Box and Whisker format that shows the median value (horizontal line), the upper and lower quartiles (boxed) and the range of the data (whiskers). The remaining "outliers" are shown as solid circles.