Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn Superoxide Dismutases

Table 2 Oligonucleotides used

Primer	Sequence (5' – 3')
H5P1 ^a	GCGGATACGCAGCAGAACAGGAAGTCCCAATGAACCTTGTCTGTAGGCTGGAGCTGCTTCG
H6P2 ^a	CGCACCACCACCGCCCAGGGGCTCCGGATGGTCATGATGGCATATGAATATCCTCCTTAG
H7P1 ^b	GGGCAGTCAATTGGTAGCGTCACCATTACTGAAACCGATATGTAGGCTGGAGCTGCTTCG
H8P2 ^b	CACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTACATATGAATATCCTCCTTAG
3xFLAG-F ^c	GGGCGGTGGTGGTGCGAGAATGGCCTGCGGCATCATTCAAGACTACAAAGACCATGACGG
3xFLAG-R2 ^c	ATGTCACGACAAAAACATTAACTCAGAGAGGGAGGATGTGCCGCATATGAATATCCTCCTTAG
3xFLAGcoli-F ^d	GGGCGGTGGTGGTGCGAGAATGGCCTGCGGCATCATTCAAGACTACAAAGACCATGACGG
3xFLAGcoli-R ^d	GTGAGCGTGGCGTTCAGCAAAAATCACTGGCGGCCACACTCATATGAATATCCTCCTTAG
240ATG-F1 ^e	GACCTTCAATCGGCCCTT
240ATG-F2 ^e	GGCCTTCTATCGGTCCCT
550F1b ^e	CCCAGTTACCGTTACGCTGCAA
550F2b ^e	TCCATTTACCACCACGCTCAAG
500STOP1R ^e	CGGGGCATCAATGGCGTTATG
500STOP2R ^e	GGAGCATCGATGGCAGCCTG
120ATGcoli-F^e	GGTTTCGTATCCGTAAAGCG
300STOPcoli-R ^e	GGGTATAGTGCTGCTGAACT
Clo-Int ^e	CTGGATATACCACCGTTGAT
Stop Clo-Int ^e	CACTCATCGCAGTACTGTT
Kan-F ^e	TGAACAAGATGGATTGCACG
Kan-R ^e	AAGAACTCGTCAAGAAGGC
O157-1For	CCGGAATTCTAAATGTAAAATCATTGCTGCC
O157-1Rev	ACAAGCTTTTATTGAATGATGCCGCAGG
O157-2For	ATCCATGGCTGCAGAACAGGAAGTCCCAAT
O157-2Rev	CAGAATTCTTATTGAATGATGCCGCAGG
PromEDLFor	TTGAATTCCCGTGTCCGTCAGCGGGG
PRromEDLRev	TTGGATCCGCCATAAAACCCTCATTAATTC

P1, P2 and 3xFLAG homolog sequences are underlined. (a) primers used to create sodC-F1/sodC-F2 null mutants, (b) primers used to create sodC null mutant; (c) primers used to insert 3xFLAG epitope in sodC-F1/sodC-F2 genes; (d) primers used to insert 3xFLAG epitope in sodC gene; (e) primers used for screening of mutant clones or in southern experiments.

ISSN: 1471-2180