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Figure 4 | BMC Microbiology

Figure 4

From: Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn Superoxide Dismutases

Figure 4

Analysis of soluble and membrane-associated proteins. Panel A: SDS-PAGE analysis of soluble (lanes 1–4) and membrane-associated proteins (lanes 5–8) from E. coli 71/18 bearing pEMBL18 (lanes 1 and 5), pO157-F1 (lanes 2 and 6), pO157-ΔCys (lanes 3 and 7), and pPLpEMBL18 (lanes 4 and 8). Panel B: Soluble (lanes 1–3) and membrane-associated epitope-tagged proteins (lanes 4–6) from E. coli ED597 (lanes 1 and 4), RG-F108 (lanes 2 and 5) and RG-F106 (lanes 3 and 6) strains were extracted from cells grown at 37°C for 24 h. Each lane was loaded with material from approximately 5 × 107CFU.

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BMC Microbiology

ISSN: 1471-2180

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