Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
Strains | ||
Lactobacillus brevis ATCC 8287 | ATCC | |
Lactobacillus acidophilus ATCC 4356 | ATCC | |
Escherichia coli DH5αF' | F' endA1 hsd17 (rk- mk+) supE44 thi-1 recA1 gyrA (NaIr) relA1Δ (lacIZYA-argF) U169 deoR [ϕ80 d lacΔ(lacZ) M15] | 58 |
Escherichia coli BL21(DE3) | F-ompT hsdS B (rB- mB-) gal dcm (DE3) | Novagen |
Plasmids | ||
pET-28a(+) | Kmr, E. coli expression vector | Novagen |
pET-28b(+) | Kmr, E. coli expression vector | Novagen |
pKTH5198 | Kmr, pET-28b(+)(NcoI/XhoI::SlpA1–435-linkerthrombin-Taghis6) | This study |
pKTH5199 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA1–435) | This study |
pKTH5200 | Kmr, pET-28b(+)(NcoI/XhoI::SlpA 146–435-linkerthrombin-Taghis6) | This study |
pKTH5201 | Kmr, pET-28b(+)(NcoI/XhoI::SlpA291–435-linkerthrombin-Taghis6) | This study |
pKTH5203 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA1–145) | This study |
pKTH5204 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA1–290) | This study |
pKTH5258 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA190–423) | This study |
pKTH5259 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA210–423) | This study |
pKTH5260 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA1–189) | This study |
pKTH5261 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA190–435) | This study |
pKTH5262 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA210–435) | This study |
pKTH5264 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA167–435) | This study |
pKTH5325 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA179–435) | This study |
pKTH5333 | Kmr, pET-28a(+)(NheI::Taghis6-linkerthrombin-SlpA149–435) | This study |