Figure 1

Observation of Xanthomonas oryzae pv. oryzae carrying green fluorescence protein. PXO99_GFP selected on PSA plates containing kanamycin (50 μg/ml) were cultured with 5 ml PS broth medium, harvested with centrifugation, washed with distilled water three times, re-suspended with 10 μl of autoclaved water, and then observed with microscope equipped with a fluorescein isothiocyanate filter (excitation filter, 450 to 490 nm; emission filter, 520 nm; dichroic mirror, 510 nm). Lefts are images with PXO99 wild type (up) and PXO99_GFP (down) strains under bright light. Rights are the images of the bacteria with illumination of UV. The bars in the left bottom of each image indicate 5 μm.