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Figure 1 | BMC Microbiology

Figure 1

From: An efficient method for visualization and growth of fluorescent Xanthomonas oryzae pv. oryzae in planta

Figure 1

Observation of Xanthomonas oryzae pv. oryzae carrying green fluorescence protein. PXO99GFP selected on PSA plates containing kanamycin (50 μg/ml) were cultured with 5 ml PS broth medium, harvested with centrifugation, washed with distilled water three times, re-suspended with 10 μl of autoclaved water, and then observed with microscope equipped with a fluorescein isothiocyanate filter (excitation filter, 450 to 490 nm; emission filter, 520 nm; dichroic mirror, 510 nm). Lefts are images with PXO99 wild type (up) and PXO99GFP (down) strains under bright light. Rights are the images of the bacteria with illumination of UV. The bars in the left bottom of each image indicate 5 μm.

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