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Table 2 Sensitivity of the LAMP assay for V. parahaemolyticus

From: Development of a loop-mediated Isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus

Strain Samples   Dilutions of cultures for the assays
    10-1 10-2 10-3 10-4 10-5
V. parahaemolyticus        
AQ4980        
  Pure cultures CFU per reaction 196.3 19.6 2.0 0.2 0.02
   LAMP + + + ± (1/3) -
   PCR + + ± (1/3) - -
  Spiked shrimps CFU per reaction 196.3 19.6 2.0 0.2 0.02
   LAMP + + + - -
   CFU per reaction 19.6 2.0 0.2 0.02 0.002
   PCR + - - - ND
  1. +, triplicate assay showed all positive.
  2. ±, triplicate assay showed both positive and negative (positive number/tested number).
  3. -, triplicate assay showed all negative.
  4. ND, Not determined.