Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli

BMC Microbiology

Table 1 Comparison of ThTP- and ATP-synthesizing activity of adenylate kinases from various sources.

Sources	ThTP synthesis (pmol min^-1 mg^-1)	ATP synthesis (pmol min^-1 mg^-1)	Ratio ATP/ThTP
AK B. stearothermophilus^a	0.2 (pH 6.5)	1.8 × 10⁶	9 × 10⁶
AK E. coli^a	35	137 × 10⁶	3.9 × 10⁶
AK1 pig skeletal muscle^b	570	1055 × 10⁶	1.8 × 10⁶
AK1 chicken skeletal muscle^c	60	155 × 10⁶	2.6 × 10⁶
Ibid.	265 (pH 10)	155 × 10⁶	0.6 × 10⁶

ThTP synthesis was measured at pH 7.5, except when indicated, as described in the Methods section. The assays for ATP synthesis were done at pH 7.5. ^a, this study; ^b, [11]; ^c, [16]

ISSN: 1471-2180