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Figure 1 | BMC Microbiology

Figure 1

From: Passive administration of monoclonal antibodies to Anthrolysin O prolong survival in mice lethally infected with Bacillus anthracis

Figure 1

A. Antibody (Ab) response of mice immunized with recombinant anthrolysin (rALO) after initial immunization at day 14 as determined by ELISA. Immunization was done with complete Freund's adjuvant. Serum dilution is represented by different fill patterns. B. Ab response after mice were boosted with same amount toxin in incomplete Freund's adjuvant measured at day 28. Individual mouse are denoted by M and numbers 1 to 10. Mice 1–5 received 1 μg of rALO and mice 6–10 received 10 μg of rALO. Pre-bleed is serum from mouse 10 before immunization and was used as control in panels A and B. Alkaline phosphatase conjugated goat anti-mouse IgG (AP-GAM-IgG) alone was used a negative control (NS: no serum) for A and B. Inset, ELISA configuration for both figures whereby rALO is absorbed on polystyrene plates and the presence of specific Ab to rALO is detected with AP-GAM-IgG.

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BMC Microbiology

ISSN: 1471-2180

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