Figure 3

Pbhsp90 expression profiles in mycelium and yeast cells, during differentiation and in the presence of oxidative agents. Total RNA (15 μg) extracted from P. brasiliensis was separated by denaturing agarose gel electrophoresis, blotted onto charged nylon membranes and probed with radioactively labelled Pbhsp90 cDNA. Panel A – mycelium (M) and yeast (Y) cells. Panel B – Mycelium cells grown at 22°C and after incubation at 36°C for up to 24 h. The membrane shown on panel B was washed to higher stringency to resolve the strong hybridization signals better. Panel C – Yeast cells incubated with 15 mM hydrogen peroxide or 1 mM menadione, a superoxide-generating reagent. In each panel, the top image represents the specific hybridization signal and the ethidium bromide stained 16S rRNA band in the bottom image.