Figure 1

ClustalW alignment of PbHSP90 and other HSP90 family proteins. The Pbhsp90 ORF was translated and aligned with Saccharomyces cerevisiae (Hsp82p, accession number [GenBank: NP_015084.1]) and Homo sapiens (HSP90β, accession number [GenBank: AAQ63401.1]) homologues using ClustalW. Shaded amino acid residues are involved in the binding of either ATP/ADP or geldanamycin in S. cerevisiae [27]. The N-terminal MEEVD motif (bold) is the binding site for the TPR domain of co-chaperones. A total of 400 out of the 706 amino acid residues are conserved across the three species.