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Table 6 Results of the real-time multiplex PCR assay for the detection of Salmonella from blind culture samples compared to the reported identification from another laboratory.

From: Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Lab. ID

Real-time multiplex PCR

Real-time Multiplex PCR Result

Reported Identification by other lab

 

aceK

fliC

sefA

sdf

  

BS1

+a (23.94)

-b

-

-

Salmonella

Gaminara

BS2

+ (24.01)c

-

+(23.01)

-

Gallinarum/Dublin

Dublin

BS3

-

-

-

-

NDd

Non-Salmonella

BS4

+ (24.16)

-

-

-

Salmonella

Branderup

BS5

+ (24.77)

-

-

-

Salmonella

Manhattan

BS6

+ (23.89)

-

-

-

Salmonella

Nottingham

BS7

-

-

-

-

ND

Non-Salmonella

BS8

+ (25.36)

-

-

-

Salmonella

Anatum

BS9

+ (22.93)

-

-

-

Salmonella

SanDiego

BS10

+ (24.02)

+ (20.40)

-

-

Typhimurium/Kentucky

Kentucky

BS11e

+ (23.10)

-

-

-

Salmonella

Non-Salmonella

BS12

+ (23.78)

-

-

-

Salmonella

London

BS13

+ (24.67)

-

-

-

Salmonella

Virchow

BS14

+ (24.79)

-

-

-

Salmonella

Livingstone

BS15

-

-

-

-

ND

Non-Salmonella

BS16

+ (22.90)

-

-

-

Salmonella

Derby

BS17

-

-

-

-

ND

Non-Salmonella

BS18

+ (23.42)

-

+ (22.03)

-

Gallinarum

Gallinarum

BS19

+ (24.33)

-

-

-

Salmonella

Saintpaul

BS20

+ (25.45)

-

-

-

Salmonella

Agona

BS21

-

-

-

-

ND

Non-Salmonella

BS22

+ (23.04)

+ (20.55)

-

-

Typhimurium/Kentucky

Typhimurium

BS23

+ (22.59)

+ (20.03)

-

-

Typhimurium/Kentucky

Typhimurium

BS24

+ (23.59)

-

-

-

Salmonella

Hadar

BS25

+ (23.49)

-

-

-

Salmonella

Heidelberg

BS26

+ (23.12)

-

-

-

Salmonella

Newport

BS27

+ (23.73)

+ (22.68)

-

-

Typhimurium/Kentucky

Typhimurium

BS28

+ (23.96)

-

-

-

Salmonella

Reading

BS29

+ (22.56)

+ (21.35)

-

-

Typhimurium/Kentucky

Typhimurium

BS30

+ (22.79)

-

-

-

Salmonella

Infantis

  1. a + = Salmonella positive by method.
  2. b – = Salmonella negative by method.
  3. c = CT values in parentheses.
  4. d ND = Salmonella not detected.
  5. e = BS11 was identified as Salmonella-positive by aceK target and confirmed by biochemical and serology testing, while the another laboratory reported that it was a non-Salmonella isolate.