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Table 6 Results of the real-time multiplex PCR assay for the detection of Salmonella from blind culture samples compared to the reported identification from another laboratory.

From: Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Lab. ID Real-time multiplex PCR Real-time Multiplex PCR Result Reported Identification by other lab
  aceK fliC sefA sdf   
BS1 +a (23.94) -b - - Salmonella Gaminara
BS2 + (24.01)c - +(23.01) - Gallinarum/Dublin Dublin
BS3 - - - - NDd Non-Salmonella
BS4 + (24.16) - - - Salmonella Branderup
BS5 + (24.77) - - - Salmonella Manhattan
BS6 + (23.89) - - - Salmonella Nottingham
BS7 - - - - ND Non-Salmonella
BS8 + (25.36) - - - Salmonella Anatum
BS9 + (22.93) - - - Salmonella SanDiego
BS10 + (24.02) + (20.40) - - Typhimurium/Kentucky Kentucky
BS11e + (23.10) - - - Salmonella Non-Salmonella
BS12 + (23.78) - - - Salmonella London
BS13 + (24.67) - - - Salmonella Virchow
BS14 + (24.79) - - - Salmonella Livingstone
BS15 - - - - ND Non-Salmonella
BS16 + (22.90) - - - Salmonella Derby
BS17 - - - - ND Non-Salmonella
BS18 + (23.42) - + (22.03) - Gallinarum Gallinarum
BS19 + (24.33) - - - Salmonella Saintpaul
BS20 + (25.45) - - - Salmonella Agona
BS21 - - - - ND Non-Salmonella
BS22 + (23.04) + (20.55) - - Typhimurium/Kentucky Typhimurium
BS23 + (22.59) + (20.03) - - Typhimurium/Kentucky Typhimurium
BS24 + (23.59) - - - Salmonella Hadar
BS25 + (23.49) - - - Salmonella Heidelberg
BS26 + (23.12) - - - Salmonella Newport
BS27 + (23.73) + (22.68) - - Typhimurium/Kentucky Typhimurium
BS28 + (23.96) - - - Salmonella Reading
BS29 + (22.56) + (21.35) - - Typhimurium/Kentucky Typhimurium
BS30 + (22.79) - - - Salmonella Infantis
  1. a + = Salmonella positive by method.
  2. b – = Salmonella negative by method.
  3. c = CT values in parentheses.
  4. d ND = Salmonella not detected.
  5. e = BS11 was identified as Salmonella-positive by aceK target and confirmed by biochemical and serology testing, while the another laboratory reported that it was a non-Salmonella isolate.