Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Table 4 Results of the real-time multiplex PCR assay compared to the traditional cultural method for the detection of Salmonella from artificially contaminated chicken skin and chicken meat samples

Food Type	Salmonella serotype	Estimated CFU by plating	ISO 6579	Real-time multiplex PCR
				aceK	fliC	sefA	sdf
Chicken skin	Enteritidis	0	-^a	-	-	-	-
		3	+^b	+ (28.14)	-	+ (27.70)	+ (26.99)
		32	+	+ (24.94)	-	+ (25.15)	+ (24.51)
		168	+	+ (22.52)	-	+ (22.32)	+ (21.68)
Chicken meat	Enteritidis	0	-	-	-	-	-
		5	+	+ (20.58)	-	+ (20.09)	+ (19.88)
		36	+	+ (20.20)	-	+ (19.96)	+ (19.57)
		222	+	+ (19.67)	-	+ (19.67)	+ (19.05)
Chicken skin	Typhimurium	0	-	-	-	-	-
		3	+	+ (24.12)	+ (23.46)	-	-
		34	+	+ (22.18)	+ (21.00)	-	-
		257	+	+ (21.95)	+ (19.57)	-	-
Chicken meat	Typhimurium	0	-	-	-	-	-
		7	+	+ (23.19)	+ (22.75)	-	-
		54	+	+ (21.39)	+ (21.19)	-	-
		305	+	+ (20.84)	+ (20.42)	-	-
Chicken skin	Kentucky	0	-	-	-	-	-
		6	+	+ (26.28)	+ (25.94)	-	-
		48	+	+ (24.25)	+ (23.70)	-	-
		280	+	+ (22.12)	+ (21.41)	-	-
Chicken meat	Kentucky	0	-	-	-	-	-
		5	+	+ (23.55)	+ (22.33)	-	-
		52	+	+ (21.15)	+ (19.93)	-	-
		277	+	+ (20.57)	+ (19.49)	-	-
Chicken skin	Bredeney	0	-	-	-	-	-
		6	+	+ (22.46)	-	-	-
		60	+	+ (21.58)	-	-	-
		397	+	+ (22.00)	-	-	-
Chicken meat	Bredeney	0	-	-	-	-	-
		9	+	+ (22.37)	-	-	-
		75	+	+ (21.69)	-	-	-
		338	+	+ (22.23)	-	-	-

ISSN: 1471-2180