Advertisement
Table 3 Oligonucleotide primers and probes used in real-time multiplex PCR assay.
| Target gene | Primer or Probe | Sequence (5'-3') | Tm (°C) | Amplicon Size (bp) | Reference or Accession No. |
|---|---|---|---|---|---|
| sefA | Forward | GTGGTTCAGGCAGCAGTTACT | 61.1 | 334 | L11008 |
| Reverse | CAGGGACATTTAGCGTTTCTTGAG | 59.7 | |||
| Probe | ROX-CAGCTCAGAATACAACATCAGCCAACTGG-BBQ | 66.2 | |||
| fliC | Forward | CCCCGCTTACAGGTGGACTAC | 60.2 | 433 | AY649721 |
| Reverse | AGCGGGTTTTCGGTGGTTGT | 63.6 | |||
| Probe | YAK-TAAAGCCGCATTGACAGCAGCAGGTG-BBQ | 69.8 | |||
| aceK | Forward | CCGCGCTGGTTGAGTGG | 62.0 | 240 | U43344 |
| Reverse | GCGGGGCGAATTTGTCTTTA | 60.3 | |||
| Probe | FAM-AACCACTGCCGAACTGTATATGGCGA-BBQ | 65.0 | |||
| sdf | Forward | AAATGTGTTTTATCTGATGCAAGAGG | 57.6 | 299 | [35] |
| Reverse | GTTCGTTCTTCTGGTACTTACGATGAC | 58.7 | |||
| Probe | Cy5-CGAATGGTGAGCAGACAACAGGCTGATTTA-BBQ | 68.4 | This study |
