Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Table 3 Oligonucleotide primers and probes used in real-time multiplex PCR assay.

Target gene	Primer or Probe	Sequence (5'-3')	Tm (°C)	Amplicon Size (bp)	Reference or Accession No.
sefA	Forward	GTGGTTCAGGCAGCAGTTACT	61.1	334	L11008
	Reverse	CAGGGACATTTAGCGTTTCTTGAG	59.7
	Probe	ROX-CAGCTCAGAATACAACATCAGCCAACTGG-BBQ	66.2
fliC	Forward	CCCCGCTTACAGGTGGACTAC	60.2	433	AY649721
	Reverse	AGCGGGTTTTCGGTGGTTGT	63.6
	Probe	YAK-TAAAGCCGCATTGACAGCAGCAGGTG-BBQ	69.8
aceK	Forward	CCGCGCTGGTTGAGTGG	62.0	240	U43344
	Reverse	GCGGGGCGAATTTGTCTTTA	60.3
	Probe	FAM-AACCACTGCCGAACTGTATATGGCGA-BBQ	65.0
sdf	Forward	AAATGTGTTTTATCTGATGCAAGAGG	57.6	299	[35]
	Reverse	GTTCGTTCTTCTGGTACTTACGATGAC	58.7
	Probe	Cy5-CGAATGGTGAGCAGACAACAGGCTGATTTA-BBQ	68.4		This study