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Table 1 Salmonella strains used in the selectivity testing of real-time multiplex PCR assay and traditional cultural method. CT values are given in parentheses.

From: Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Salmonella serotypes ISO 6579 Real-time multiplex PCR
   aceK fliC sefA sdf
Agona +a + (23.31) -b - -
Anatum + + (21.67) - - -
Braenderup + + (21.24) - - -
Bredeney + + (21.71) - - -
Derby + + (22.79) - - -
Dublin (n = 7)c + + (17.63) - + (19.35) -
Enteritidis (n = 11) + + (21.35) - + (23.36) + (23.67)
Gallinarum (n = 3) + + (26.87) - + (27.83) -
Goldcoast + + (24.35) - - -
Hadar + + (19.68) - - -
Heidelberg + + (21.12) - - -
Infantis + + (23.34) - - -
Kentucky (n = 8) + + (18.59) + (21.65) - -
Livingstone + + (21.90) - - -
London + + (19.92) - - -
Manhattan + + (17.53) - - -
Newport + + (18.04) - - -
Nottingham + + (21.89) - - -
Panama + + (19.54) - - -
Saintpaul + + (21.06) - - -
Senftenberg + + (21.01) - - -
Stanley + + (19.23) - - -
Typhimurium (n = 10) + + (19.77) + (22.45) - -
Uganda + + (20.21) - - -
Virchow + + (22.52) - - -
Gaminara + + (21.03) - - -
  1. a + = Salmonella positive by the method.
  2. b – = Salmonella negative by the method.
  3. c = Mean CT values are given when n > 1.