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Table 1 Salmonella strains used in the selectivity testing of real-time multiplex PCR assay and traditional cultural method. CT values are given in parentheses.

From: Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Salmonella serotypes

ISO 6579

Real-time multiplex PCR

  

aceK

fliC

sefA

sdf

Agona

+a

+ (23.31)

-b

-

-

Anatum

+

+ (21.67)

-

-

-

Braenderup

+

+ (21.24)

-

-

-

Bredeney

+

+ (21.71)

-

-

-

Derby

+

+ (22.79)

-

-

-

Dublin (n = 7)c

+

+ (17.63)

-

+ (19.35)

-

Enteritidis (n = 11)

+

+ (21.35)

-

+ (23.36)

+ (23.67)

Gallinarum (n = 3)

+

+ (26.87)

-

+ (27.83)

-

Goldcoast

+

+ (24.35)

-

-

-

Hadar

+

+ (19.68)

-

-

-

Heidelberg

+

+ (21.12)

-

-

-

Infantis

+

+ (23.34)

-

-

-

Kentucky (n = 8)

+

+ (18.59)

+ (21.65)

-

-

Livingstone

+

+ (21.90)

-

-

-

London

+

+ (19.92)

-

-

-

Manhattan

+

+ (17.53)

-

-

-

Newport

+

+ (18.04)

-

-

-

Nottingham

+

+ (21.89)

-

-

-

Panama

+

+ (19.54)

-

-

-

Saintpaul

+

+ (21.06)

-

-

-

Senftenberg

+

+ (21.01)

-

-

-

Stanley

+

+ (19.23)

-

-

-

Typhimurium (n = 10)

+

+ (19.77)

+ (22.45)

-

-

Uganda

+

+ (20.21)

-

-

-

Virchow

+

+ (22.52)

-

-

-

Gaminara

+

+ (21.03)

-

-

-

  1. a + = Salmonella positive by the method.
  2. b – = Salmonella negative by the method.
  3. c = Mean CT values are given when n > 1.