Figure 4

Production of TDH by the Δ hfq strain. (A) Detection of TDH in cell lysates from mid-log phase (OD₆₀₀ = 0.6) and stationary phase (OD₆₀₀ = 1.5) cultures. (B) Detection of TDH in mid-log phase cell lysates from the parent strain and derivatives. (C) Quantification of tdh mRNA. Fold change in the gene transcription level from mid-log phase in the Δhfq strain versus the parent strain, as measured by quantitative real-time RT-PCR. Data analysis was as previously described [42, 43]. Data are expressed as means ± SDs of 5 independent experiments. *, p < 0.05.