Table 2 Bacterial plasmids used in this work

pACYC184

Cm^r Tet^r General cloning vector

[35]

pBSKII+

Ap^r ColE1 replicon

Stratagene

pBR322

Ap^r Cloning vector

[36]

pHek6

Apr hek gene in Pbskii+

[14]

pHP45Ω

Ap^r Spc^r pMB1 replicon

[37]

PInvA

Ap^r invA gene with 500 bp flanking DNA cloned into pBSKII+

This study

pInvAKO

Ap^r invA::cat gene with 500 bp flanking DNA cloned into pBSKII+

This study

pKOBEGA

Ap^r Temperature-sensitive plasmid carrying the red and gam genes of λ-phage under control P _araBAD promoter

[32]

pMALc2

Ap^r MBP fusion vector

New England Biolabs

pML1

Ap^r pagN ORF inserted into pTrc99a

This study

pML4

Ap^r pagN ORF flanked by NcoI and BamHI sites inserted into pBSKII+

This study

pML7

Ap^r pagN ORF, excluding the DNA corresponding to the signal sequence of the PagN protein, inserted into pMAL-c2

This study

pML10

Ap^r pagN gene inserted into pBR322

This study

pPagN2.3

Ap^r pagN gene with 500 bp flanking DNA cloned into pBSKII+

This study

pPagNKO

Ap^r Spc^r pagN::spc

This study

pTrc99a

Ap^r Cloning vector with an IPTG-inducible P _trc promoter

[38]