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Table 4 Differential amplification kinetics for the SNP alleles.

From: Assignment of Streptococcus agalactiae isolates to clonal complexes using a small set of single nucleotide polymorphisms

SNP ΔCT ± SD (allele 1) ΔCT ± SD (allele 2) ΔΔCT
glnA36 7.6 ± 1.0 (C) 7.5 ± 1.2 (T) 15.1
glnA429 10.9 ± 3.0 (C) 3.9 ± 2.5 (T) 14.8
glcK180 8.5 ± 2.4 (A) 11.1 ± 1.7 (G) 19.6
adhP111 14.9 ± 1.6 (G) 9.5 ± 2.0 (A) 24.4
Atr351 (safety net) 2.1 ± 0.6 (T) 6.6 ± 0.5 (A) 8.7
  1. The ΔCT is the CT for the reaction containing the mis-matched primer – the CT for the reaction containing the matched primer. SD is the standard deviation. The ΔΔCT is the difference between the two ΔCT values and represents the SNP signal.