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Table 1 Sequences of primers used in kinetic PCR reactions.

From: Assignment of Streptococcus agalactiae isolates to clonal complexes using a small set of single nucleotide polymorphisms

SNP

Primer Name

Primer Sequence (5' – 3')

glnA36

glnA36-C

ATATCCTGATTTAGATACTTGGATTCTC

 

glnA36-T

ATATCCTGATTTAGATACTTGGATTCTT

 

glnA36-Rev

TCTCCTTCTGCTGTATAGATATCACA

glnA4291

glnA429-G1

TTGTTTTAACAACCAATTTAAATAATTGAATC

 

glnA429-A

TTGTTTTAACAACCAATTTAAATAATTGAATT

 

glnA429-For

AAGTAGCAGTTGGACAGGATGAAA

glcK180

glcK180-A

GGCTGATACTCAAGAAGTAGGTTAA

 

glcK180-G

GGCTGATACTCAAGAAGTAGGTTAG

 

glcK180-Rev

ACCAAGTGCTGCAACATTAGC

adhP111

adhP111-G

TTGCATGGTTCTTTGAATGG

 

adhP111-A

TTGCATGGTTCTTTGAATGA

 

adhP111-T

TTGCATGGTTCTTTGAATGT

 

adhP111-Rev

CAAAGCGTCTCACGTCCTGT

atr351

atr351-A

GTTGTTGTTGTCCTCCAGATAAGCTA

 

atr351-T

GTTGTTGTTGTCCTCCAGATAAGCTT

 

atr351-Rev

GGACTCAAAGAGAAGGCTAATGCT

  1. The allele specific primers are named in accordance with their specificities, while the common primers are names in accordance with their orientation with respect to the coding DNA sequences.
  2. 1The glnA429 allele specific primers are in the reverse direction, hence the inconsistency between the alleles and the 3' terminal nucleotides.
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BMC Microbiology

ISSN: 1471-2180

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