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Table 1 Bacterial strains and plasmids used in this study

From: Detection of quorum-sensing-related molecules in Vibrio scophthalmi

Strains or Plasmids Characteristics Reference or source
Strains   
Vibrio scophthalmi A089 Turbot isolate, CECT 4638T [18]
Vibrio scophthalmi A102 Turbot isolate [18]
Vibrio scophthalmi A089-aiiA strain A089 strain with the aiiA gene cloned into pACYC184 plasmid, Clr This study
Vibrio scophthalmi A102-aiiA strain A102 strain with the aiiA gene cloned into pACYC184 plasmid, Clr This study
E. coli DH5α F-Φ80d lacZΔM15endA1hsdR17(rk- mk-) supE44 thi-1 gyrA96Δ(lacZYA-argF) [37]
E. coli HB101 (pRK2073) Mobilizing strain; Spr Biomedal
E. coli One Shot cells Used for transformation Experiments Invitrogen
Sensor strains   
A. tumefaciens NTL4(pZLR4) AHLs sensor strain, Gmr [34]
C. violaceum CV026 AHLs sensor strain [25]
V. harveyi BB866 AI-1 indicator strain, BB120 luxP::Tn5 [2]
V. harveyi BB170 AI-2 indicator strain, BB120 luxN::Tn5 [42]
V. harveyi BB120 wild-type strain [2]
P. putida F117 (pAS-C8) AHLs sensor strain, Gmr [27]
P. putida F117 (pKR-C12) AHLs sensor strain, Gmr [27]
Plasmids   
pCR2.1-TOPO TA cloning vector; Ampr, Kmr Invitrogen
pGEM-T-easy TA cloning vector; Ampr Promega
pACYC184 Broad-host range expression vector, Tcr, Clr [36]
pACYC184-aiiA Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Clr This study
pME6863 Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Tcr [4]
  1. Antibiotic abbreviations used in this study: Ampicillin (Amp), Kanamycin (Km), Spectinomycin (Sp), Chloramfenicol (Cm), Trimethoprim (Tri), Tetracycline (Tc); Spanish Type Culture Collection (CECT).