Table 1 Bacterial strains and plasmids used in this study

Strains

Vibrio scophthalmi A089

Turbot isolate, CECT 4638T

[18]

Vibrio scophthalmi A102

Turbot isolate

[18]

Vibrio scophthalmi A089-aiiA strain

A089 strain with the aiiA gene cloned into pACYC184 plasmid, Cl^r

This study

Vibrio scophthalmi A102-aiiA strain

A102 strain with the aiiA gene cloned into pACYC184 plasmid, Cl^r

This study

E. coli DH5α

F^-Φ80d lacZΔM15endA1hsdR17(r_k^- m_k^-) supE44 thi-1 gyrA96Δ(lacZYA-argF)

[37]

E. coli HB101 (pRK2073)

Mobilizing strain; Sp^r

Biomedal

E. coli One Shot cells

Used for transformation Experiments

Invitrogen

Sensor strains

A. tumefaciens NTL4(pZLR4)

AHLs sensor strain, Gm^r

[34]

C. violaceum CV026

AHLs sensor strain

[25]

V. harveyi BB866

AI-1 indicator strain, BB120 luxP::Tn5

[2]

V. harveyi BB170

AI-2 indicator strain, BB120 luxN::Tn5

[42]

V. harveyi BB120

wild-type strain

[2]

P. putida F117 (pAS-C8)

AHLs sensor strain, Gm^r

[27]

P. putida F117 (pKR-C12)

AHLs sensor strain, Gm^r

[27]

Plasmids

pCR2.1-TOPO

TA cloning vector; Amp^r, Km^r

Invitrogen

pGEM-T-easy

TA cloning vector; Amp^r

Promega

pACYC184

Broad-host range expression vector, Tc^r, Cl^r

[36]

pACYC184-aiiA

Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Cl^r

This study

pME6863

Broad-host range expression vector carrying the aiiA gene from Bacillus cereus strain A24; Tc^r

[4]