Chromatograph obtained from the lower AHL signalling spot from the TLC. A peak of 300.2414 was obtained with a Mariner TOF MS, whose exact mass was later confirmed as m/z 300.2167 after HPLC and mass spectrometry with electrospray ionisation analysis. This molecule matched the empirical formula C16H29NO4 (with an error of 0.39 ppm) for M and hypothetically corresponds to a molecular structure of a hydroxylated-C12HSL. A peak of 282.2256 was also observed which could represent loss of OH group due to fragmentation of the molecule, and a 599.4826 peak, an aggregation of two molecules. Other peaks were originated from components of the medium used for growing the bacterium.