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Table 4 Primers for amplification of DNA fragments encoding each Ig-binding domains

From: Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Name Description Sequence(5' → 3')
PA(A)-Uxk Sense terminal primer of PA(A) C CTG GGT ACCTCT AGA* GCT GAC AAC AAC TTC AAC
PA(D)-Uxl Sense terminal primer of PA(D) TAT GGT ACC TCT AGAGCT GAC GCT CAG CAG AAC
PA(A/D)-Dxk Antisense random primer of PA(A/D) ACT GGT ACC TCT AGA (0N, 3N, 6N, 9N) ** TTT CGG AGC CTG AGA TTC
PG-Uxk Sense terminal primer of PG GCG GGT ACC TCT AGAACC TAC AAA CTG GTT ATC
PG-Dxk Antisense random primer of PG TCA GGT ACCTCT AGA (0N, 3N, 6N, 9N) TTC GGT AAC GGT GAA GGT
PL-Uxk Sense terminal primer of PL GCG GGT ACCTCT AGAAAA GAA AAA ACC CCG GAA
PL-Dxk Antisense random primer of PL TGC GGT ACC TCT AGA (0N, 3N, 6N, 9N) ACC AGC GAA TTT GAT GTT CAG
  1. *: Italic and black parts represent Xba I recognition sites; **: Underlined parts represent the random linking sequence; 0N, 3N, 6N and 9N: the sequence of random linking peptides composed of non, three, six and nine nucleotides.