Combinatorial form of single domains | PA(A)-PL-PL9N | PA(A)6N-PL9N | PA(A)-PL3N | PA(A)9N-PL3N | PA(A)9N-PL |
---|---|---|---|---|---|
Nucleotide sequence of random linking peptide | TAC TGG TTG | AAA CTA GCT AAC AAC | TTG | GGT GAG ATG CAC | GAC TTT ATT |
Amino acid sequence of random linking peptide | Y W L | K L A N N | L | G E M H | D F I |
Number of clones selected with hIgM (11*) | 4 | 2 | 3 | 1 | 1 |
Number of clones selected with hIgA (10) | 5 | 2 | 2 | 1 | 0 |