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Table 3 Sequences of random linking peptides in PA(A)-PL structure and their distribution in hIgM and hIgA selected libraries

From: Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Combinatorial form of single domains

PA(A)-PL-PL9N

PA(A)6N-PL9N

PA(A)-PL3N

PA(A)9N-PL3N

PA(A)9N-PL

Nucleotide sequence of random linking peptide

TAC TGG TTG

AAA CTA GCT AAC AAC

TTG

GGT GAG ATG CAC

GAC TTT ATT

Amino acid sequence of random linking peptide

Y W L

K L A N N

L

G E M H

D F I

Number of clones selected with hIgM (11*)

4

2

3

1

1

Number of clones selected with hIgA (10)

5

2

2

1

0

  1. *: Number of sequenced phage clones; Underlined and italic parts represent the nucleotide and amino acid sequences of linking peptide followed the second domain. 3N, 6N and 9N: the sequence of random linking peptides composed of three, six and nine nucleotides. Single letter abbreviations of amino acids: A, Ala; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; W, Trp; Y, Tyr.