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Table 3 Sequences of random linking peptides in PA(A)-PL structure and their distribution in hIgM and hIgA selected libraries

From: Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Combinatorial form of single domains PA(A)-PL-PL9N PA(A)6N-PL9N PA(A)-PL3N PA(A)9N-PL3N PA(A)9N-PL
Nucleotide sequence of random linking peptide TAC TGG TTG AAA CTA GCT AAC AAC TTG GGT GAG ATG CAC GAC TTT ATT
Amino acid sequence of random linking peptide Y W L K L A N N L G E M H D F I
Number of clones selected with hIgM (11*) 4 2 3 1 1
Number of clones selected with hIgA (10) 5 2 2 1 0
  1. *: Number of sequenced phage clones; Underlined and italic parts represent the nucleotide and amino acid sequences of linking peptide followed the second domain. 3N, 6N and 9N: the sequence of random linking peptides composed of three, six and nine nucleotides. Single letter abbreviations of amino acids: A, Ala; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; W, Trp; Y, Tyr.
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