Combinatorial form of single domains
|
PA(A)-PL-PL9N
|
PA(A)6N-PL9N
|
PA(A)-PL3N
|
PA(A)9N-PL3N
|
PA(A)9N-PL
|
---|
Nucleotide sequence of random linking peptide
|
TAC TGG TTG
|
AAA CTA GCT AAC AAC
|
TTG
|
GGT GAG ATG CAC
|
GAC TTT ATT
|
Amino acid sequence of random linking peptide
|
Y W L
|
K L A N N
|
L
|
G E M H
|
D F I
|
Number of clones selected with hIgM (11*)
|
4
|
2
|
3
|
1
|
1
|
Number of clones selected with hIgA (10)
|
5
|
2
|
2
|
1
|
0
|
- *: Number of sequenced phage clones; Underlined and italic parts represent the nucleotide and amino acid sequences of linking peptide followed the second domain. 3N, 6N and 9N: the sequence of random linking peptides composed of three, six and nine nucleotides. Single letter abbreviations of amino acids: A, Ala; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; W, Trp; Y, Tyr.