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Table 2 Sequences of random linking peptides in PA(A)-PG structure and their distribution in hIgG and hIgG1-Fc selected libraries

From: Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Combinatorial form of single domains PA(A)-PG PA(A)-PG9N PA(A)-PG3N PA(A)6N-PG3N PA(A)6N-PG PA(A)3N-PG-PA(A)
Nucleotide sequence of random linking peptide - AGC TTA CAC CAC ACC TCG ACC CAC TCA CCA
Amino acid sequence of random linking peptide - S L H H T S T H S P
Number of clones Selected with hIgG (10*) 2 5 2 1 0 0
Number of clones Selected with hIgG1-Fc (10) 7 0 0 0 2 1
  1. *: Number of sequenced phage clones; Underlined and italic parts represent the nucleotide and amino acid sequences of linking peptide followed the second domain. 3N, 6N and 9N: the sequence of random linking peptides composed of three, six and nine nucleotides. Single letter abbreviations of amino acids: H, His; L, Leu; P, Pro; S, Ser; T, Thr.