Figure 1

Detection of inserted fragments of phage clones in each round of hIgG selection library by PCR. PCR products were analyzed by electrophoresis in 1.2% agarose gel and detected by staining with ethidium bromide. No. 1 to 22: randomly picked phage clones; M: DL2000 Marker; C: positive control (pCANTAB5S vector); B: negative control (blank culture medium); I, II, III, IV: the first to the fourth round of selection respectively.