Figure 5From: Generation of a restriction minus enteropathogenic Escherichia coli E2348/69 strain that is efficiently transformed with large, low copy plasmidsNH4 is unaffected in T3S-mediated host cell intoxication. E2348/69 and NH4 were used to infect HEp-2 tissue culture cells. Actin (green) and EPEC bacteria (red) were detected after staining with Alexa 488 phalloidin and rabbit anti-EPEC polyclonal anti-sera, followed by anti-rabbit antibody labeled with Cy3.Back to article page