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Figure 9 | BMC Microbiology

Figure 9

From: Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of priA and its role in intracellular replication

Figure 9

Immunofluorescence analysis of HeLa cells during infection. HeLa cells were infected with B1940, B1940ΩpriA or B1940ΩpriA/priA+ as indicated. Gentamicin selection was used to eliminate extracellular bacteria. Images were taken 7 h after infection. Antibodies against N. meningitidis were used before permeabilization with saponin in combination with a secondary antibody conjugated with Cy5 and, after permeabilization, with a secondary TRITC-conjugated antibody. Intracellular bacteria were therefore red while extracellular bacteria were expected to be purple (the combination of TRITC and Cy5 signal). In the images all bacteria were intracellular. To detect cellular markers we used anti-LAMP1 and a secondary antibody conjugated with FITC.

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