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Figure 6 | BMC Microbiology

Figure 6

From: Reverse transcriptase-PCR differential display analysis of meningococcal transcripts during infection of human cells: Up-regulation of priA and its role in intracellular replication

Figure 6

Effects of priA inactivation on meningococcal viability. Meningococcal strains B1940, B1940ΩpriA and B1940ΩpriA/priA+ were grown to either middle (0.5 OD600 nm) or late (1.0 OD600 nm) logarithmic phase and then viability was determined by using the Live/Dead BacLight bacterial viability kit (Molecular Probe). (A) Representative images of Live/Dead-stained meningococci: live bacteria fluoresce green, while dead bacteria fluoresce red. (B) Percentages of non-viable meningococci were equal to ratios between bacteria staining positive with propidium iodide (red) and the total stained (red + green) bacteria. Data are shown as mean ± standard deviation of three experiments. Statistically significant difference between values from B1940 (or B1940ΩpriA/priA+) and B1940ΩpriA (asterisk) is declared at a p value < 0.05.

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