Table 1 Strains and plasmids used in this study
Strains | Genotype | Source or Reference |
|---|---|---|
Escherichia coli | ||
DH5α | F-, Δ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1 | Laboratory collection |
|  | hsdR17(rk-, mk+), phoA, supE44, λ -, thi-1, gyrA96, relA1 |  |
Bacillus subtilis | Â | Â |
168 | trpC2 | Laboratory collection |
BFS2842 | trpC2 spx::lacZ erm | Functional analysis projecta |
BSY2530 | trpC2 amyE:: pX vector cat | This work |
BSY2531 | trpC2 amyE:: (pxyl-spx10A-HA) cat | This work |
BSY2533 | trpC2 amyE:: (pxyl-spx13A-HA) cat | This work |
BSY2534 | trpC2 amyE:: (pxyl-spx-HA) cat | This work |
BSY2535 | trpC2 amyE:: (pxyl-spx10A13A-HA) cat | This work |
BSY4260 | trpC2 clpX::aphA-3 spx::lacZ erm | This work |
BSY4546 | trpC2 msrA::lacZ cat | This work |
BSY5000 | trpC2 spx::aphA-3 | This work |
BSY5051 | trpC2 spx::aphA-3 thrC::(pxyl-spx) spc | This work |
BSY5052 | trpC2 spx::aphA-3 thrC::(pxyl-spx10A13A) spc | This work |
BSY5531 | trpC2 spx::aphA-3 amyE:: (pxyl-spx10A-HA) cat | This work |
BSY5533 | trpC2 spx::aphA-3 amyE:: (pxyl-spx13A-HA) cat | This work |
BSY5534 | trpC2 spx::aphA-3 amyE:: (pxyl-spx-HA) cat | This work |
BSY5535 | trpC2 spx::aphA-3 amyE:: (pxyl-spx10A13A-HA) cat | This work |
BSY6000 | trpC2 clpX::aphA-3 | This work |
Plasmids | Description | Source or Reference |
pDIA5307 | lacZ transcriptional fusion vector, AmpR CamR | [37] |
pDG784 | aphA-3 gene delivery vector, KanR | [38] |
pXT | gene expression vector AmpRSpcR | [40] |
pX | gene expression vector AmpRCamR | [41] |
pCHY109 | pXT spx AmpRSpcR | This work |
pCHY110 | pXT spx10A13A AmpRSpcR | This work |
pCHY111 | pX spx10A-HA AmpRCamR | This work |
pCHY113 | pX spx13A-HA AmpRCamR | This work |
pCHY115 | pX spx10A13A-HA AmpRCamR | This work |
pCHY253 | pX spx-HA AmpRCamR | This work |
pCHY4546 | pDIA5307 msrA::lacZ AmpR CamR | This work |