Characterization of new IS elements and studies of their dispersion in two subspecies of Leifsonia xyli

BMC Microbiology

Table 1 Details of Lxc-IS elements.

*Lxc*-IS	IS length (bp)	Tpase length (aa)^a	IRs	DR (host sequence)	Minimal n^o of copies	IS family^b	Most related *Lxx*-IS
IS1237	899/798	218	5' GAGGTGGTTTCAGTAGTCG 3' GAGATGGTTTCAGTAGTCG^c	TTA (TAA)	26	IS5	ISLxx6
ISLxc1	2631	262 (istB)	5' TGTCTGTGTCACTGTTAGCGGGCTCCG GGTTGTCGGAGTTGTCTGGCCCCGGCG 3' TGACTATGTCAAGGCTGGCGGGGACCG GGTTGTCGAAGTTAGTTGGCCCCAGCG	fATCGCC	2	IS21	ISLxx3
ISLxc2	1105	332	5' TGTGTTTCTCAGGGACGTTGGT 3' TGTGCTGCTCAGGGACGTTGGT	NCTAGN	16	IS481	ISLxx4
ISLxc3	1511	351	5' CTTGGGTTCTAACGGTCGTTGCAACA 3' GGCGGATTCTAGTGGTCGTTGCAACA	-	8	IS30	ISLxx5
ISLxc4	1311/896	380	5' GGGTGGGTTCTAACAGTGGTCGCAACAC 3' GGGTGGGTTCTAACAGTGGTCGCAACAC	-	5	IS30	ISLxx5

N – not complementary nucleotides
^a – assuming predicted features for regulation of transposition at translational level are overcome.
^b – according to Chandler & Mahillon family classification [11].
^c – three IS1237 copies have a G instead of A in the 3' IR, making perfectly complementary IRs.

ISSN: 1471-2180