Table 1 S. Typhimurium mutants that exhibited different type 1 fimbrial phenotypes than the parental strain

(agar, broth)

Fimbrial biosynthesis and regulation

K2

-, -

fimZ

Positive regulator of type 1 fimbriae major subunit gene fimA

K14

-, -

fimY

Positive regulator of type 1 fimbriae major subunit gene fimA

K40

-, -

fimA

Major subunit of type 1 fimbriae

K66

-, -

fimD

Fimbrial usher protein of FimA

K75

-, -

fimC

Fimbrial chaperone protein of FimA

K44

-, -

fimH

Adhesin of type 1 fimbriae

K70

+, +

lpfB

Fimbrial chaperone protein of long polar fimbriae

K61

+, +

pefI

Regulator of plasmid-encoded fimbriae

K23

+, +

stbC

Fimbrial usher protein of Stb fimbriae

Putative cytoplasmic protein

K1

+, +

STM1078

Putative cytoplasmic protein

K28

+, +

STM4529

Putative cytoplasmic protein

Global regulator

K46

-, -

lrp

Leucine-responsive regulatory protein

K15

-, -

hupA

Histone-like DNA binding protein

Membrane associated protein

K17,

+, +

yhjV

Putative transporter protein

K39

-, -

STM1128

Putative sodium/glucose cotransporter

K45

-, -

yihO

Putative glycoside-pentoside-hexuronide (GPH) family transport protein

K50

+, +

STM2532

Putative inner membrane lipoprotein

K52

+, +

STM2486

Putative inner membrane protein

K54

-, -

yjiH

Putative inner membrane protein

K72

+, +

mgtB

Mg²⁺ transporter protein

K30

+, +

narK

Nitrate/nitrite transporter protein

Cell envelope associated protein

K60

+, +

ytfB

Putative cell envelope opacity-associated protein A

Periplasmic protein

K69

+, +

ydbH

Putative periplasmic protein

Ribosome modulation factor

K56

-, -

rmf

Ribosome modulation factor

Type III secretion system

K55

-, -

ssaB

Effector protein of type III secretion system

K59

+, +

invB

Chaperone protein for SopE/SopE2

K63

-, -

ssaV

Apparatus protein for intracellular trafficking and secretion

Prophage-derived protein

K11

+, +

STM4200

Putative phage tail protein H

K33

+, +

sseI

Gifsy-2 prophage putative type III secreted effector protein

K19

-, -

STM1041

Gifsy-2 prophage probable minor tail protein

Sensor protein

K51

+, +

ssrA

Sensor component of type III secretion system

K58

+, +

yehU

Sensor component

Transcription termination factor

K73

-, -

nusA

Transcription termination factor

Enzymes

K3

-, -

cafA

Endoribonuclease

K5

+, +

res

Subunit of type III restriction-modification enzyme

K8

+, +

STM4467

Putative arginine deiminase

K16

+, +

STM1627

Alcohol dehydrogenase class III

K31

+, +

STM2446

Putative iron-dependent peroxidase

K41

-, -

nuoE

NADH dehydrogenase I chain E

K37

+, +

yafA

Putative hydrolase

K43

-, -

miaA

Isopentenylpyrophosphate transferase

K38

-, -

prC

C-terminal protease for penicillin binding protein

K47

-, -

atpH

Membrane-bound ATP synthase

K48

+, +

ubiB

NAD(P)H-flavin reductase

K49

-, -

ydiF

Putative acetyl-CoA: acetoacetyl-CoA transferase

K53

+, +

tdK

Thymidine kinase

K65

+, +

pps

Phosphoenolpyruvate synthase

K57

+, +

ybjX

Putative enzyme with unknown function

K67

+, +

pncA

Nicotinamidase/pyrazinamidase

K68

+, +

rfaB

Lipopolysaccharide 1,6-galactosyltransferase

K71

+, +

xapB

Xanthosine permease

K64

+, +

STM1940

Putative cell wall-associated hydrolase

K74

+, +

cbiA

Cobyrinic acid a, c-diamide synthase

K42

-, -

aroA

3-enolpyruvylshikimate-5-phosphate synthetase