TY - JOUR AU - Stirrett, Karen L. AU - Ferreras, Julian A. AU - Rossi, Sebastian M. AU - Moy, Richard L. AU - Fonseca, Fabio V. AU - Quadri, Luis EN PY - 2008 DA - 2008/07/21 TI - A multicopy suppressor screening approach as a means to identify antibiotic resistance determinant candidates in Yersinia pestis JO - BMC Microbiology SP - 122 VL - 8 IS - 1 AB - Yersinia pestis is the causative agent of plague and a potential agent of bioterrorism and biowarfare. The plague biothreat and the emergence of multidrug-resistant plague underscore the need to increase our understanding of the intrinsic potential of Y. pestis for developing antimicrobial resistance and to anticipate the mechanisms of resistance that may emerge in Y. pestis. Identification of Y. pestis genes that, when overexpressed, are capable of reducing antibiotic susceptibility is a useful strategy to expose genes that this pathogen may rely upon to evolve antibiotic resistance via a vertical modality. In this study, we explored the use of a multicopy suppressor, Escherichia coli host-based screening approach as a means to expose antibiotic resistance determinant candidates in Y. pestis. SN - 1471-2180 UR - https://doi.org/10.1186/1471-2180-8-122 DO - 10.1186/1471-2180-8-122 ID - Stirrett2008 ER -