Table 1 Purification of Brucella suis 1330 urease.
From: Enzymatic, immunological and phylogenetic characterization of Brucella suis urease
Purification step | Protein (mg) | Total activity (μmol/min) | Enzyme recovery (%) | Specific activity (μmol urea/min per mg of protein) | Purification (fold) |
|---|---|---|---|---|---|
Crude extract | 498 | 5229 | 100 | 10.5 | 1 |
Q sepharose | 31 | 3472 | 66 | 112 | 10.6 |
Source PHE | 1.2 | 648 | 12 | 540 | 51.4 |