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Figure 3 | BMC Microbiology

Figure 3

From: Enzymatic, immunological and phylogenetic characterization of Brucella suis urease

Figure 3

5–10% polyacrylamide gradient nondenaturing gel electroforesis. Lane 1, albumin from chicken egg white, 45,000 Da; lane 2, albumin from bovine serum, 66,000 Da (monomer) and 132,000 Da (dimer); lane 3, jack bean urease, 272,000 Da (trimer), and 545,000 Da (hexamer). Lanes 1–3 were stained with Coomassie blue. Lane 4, jack bean urease; lane 5, Brucella suis urease (157,000 Da ± 7010). Determination of urease activitiy was performed as described in Methods section. Four different gels were done to calculate the molecular weight of Brucella suis urease.

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