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Table 1 Analysis of recombinants.

From: Tuberculosis vaccine strain Mycobacterium bovis BCG Russia is a natural recA mutant

BCG substrain

no. of HygR colonies

no. of analyzed HygR

transformants

spontaneously HygR

   

homologous recombinants

illegitimate recombinants

 

recA+ substrains

     

   Pasteur

54

11

2

3

6

   Frappier

90

9

2

1

6

   Denmark

377

21

10

2

9

Σ recA+

521

41

14

6

21

recA- substrain

     

   Russia

72

23

0

16

7

  1. Analysis of recombinants in BCG substrains Pasteur, Frappier, Denmark, and Russia following transformation with recA targeting plasmid pGEM7-recA::3xstop-hsp60-sacB-hyg-aph
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BMC Microbiology

ISSN: 1471-2180

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