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Table 2 Primers for qPCR

From: Pleiotropic phenotypes of a Yersinia enterocolitica flhD mutant include reduced lethality in a chicken embryo model

Gene Forward primer Reverse primer
dnaE 5'-AATCACACTCTGCCGCTTATG-3' 5'-CCATCCGCCAGCACTCAT-3'
polA 5'-GCTGGCTTGCGGATGTAGAT-3' 5'-AGCACGGCGGTCACTTCA-3'
gapA 5'-CCATCCGTGTTACCGCAGAG-3' 5'-TCTTAGCACCAGCAGCAATGT-3'
fleC 5'-GTAGTAGCGGTTTTCAGTGCTGT-3 5'-CCGGTGGTATTTCAGGTGAT-3'
hutI 5'-ACTCGCAGGTGTCACTTG-3' 5'-GAGCCAGTAAGCCAATTCAG-3'
hutU 5'-CGATATTCTTCCCAGCTCCA-3' 5'-GCAACAGATTTTGATGATGCG-3'
carA 5'-AAGGCAGTTGGACTCTGGAA-3' 5'-CCGTTGGATAAGAAAATGCC-3'
carB 5'-GTTAATTGGCGGCATTATGG-3' 5'-GACTTCAATCAGGTAAACTTC-3'
pyrB 5'-CCGATTGTCGTTTGAAACCT-3' 5'-ATTGACCACCGGCACATTGCC-3'
deoB 5'-GCTGACTGAAGGCGGTTACAAT-3' 5'-GGTGCTGGCGGTTCAACTG-3'
acrE 5'-AGATTCTGGCTAACGACTACG-3' 5'-ATCACCTGAGCGATGTATGG-3'
oppA1 5'-TCAAGAACAGCCAGAAGCCCTA-3' 5'-AAGCGAGCGTGGTTGGAATAC-3'
oppB1 5'-TGGCGGTGATAGTGGATT-3' 5'-TGATCTCGGCAATAACTTCC-3'
oppC1 5'-ACCAAGATCCGTATGTCATT-3' 5'-TACCGAGCCAGTTAGTGT-3'
oppDF 5'-TGAGATTATCCAGTGCCTTGA-3' 5'-TAGTCAGAACCGCCATAGC-3'
oppB2 5'-GCATTCCTACTGGCGGTGATT-3' 5'-AGGACTAACAGAGGAGCAACGA-3'
dppB1 5'-CCTTACTGACAGTGATTGC-3' 5'-ATAATGGCGGTGGTATCG-3'
dppB2 5'-ACCATTCCTCTCGCCGTTAT-3' 5'-GACCACAATCACTCGCATCC-3'
  1. Column 1 presents the genes that were analyzed.
  2. Columns 2 and 3 indicate the primers that were used for the qPCR reaction. Sequence information was obtained from Sanger [22].